Quiz on Enzyme Kinetics

A simple quiz with graphs and some real life problems. Let’s see if you can solve it.


General biochemistry:

A simple quiz is presented to test your enzyme kinetics knowledge.

Answer the questions with respect to the graph given in that question.

Please note: More than one question might be correct in the questions so answer them accordingly.
In a fermenter, scientist observed some kind of inhibition of its enzyme used to convert its substrate to a product. He observed a graph as shown below when he compared the results with the standard results. Please answer the questions given below to solve his mystery.

1. Graph denotes which kind of plot?
1. Michaelis-Menten plot
2. Double reciprocal plot
3. X-Y plot
4. None of these
2. Inhibitor is causing which type of inhibition?
1. Reversible
2. Competitive
3. Non- competitive
4. Mixed
3. What is the effect of inhibitor on Vmax of the reaction?
1. Same
2. Decrease
3. Increase
4. Data insufficient
4. What is the effect of inhibitor on the Km of the reaction?
1. Same
2. Decrease
3. Increase
4. Data insufficient
5. How can he solve the problem?

a) Decrease substrate concentration

b) Maintain the substrate concentration

c) Increase substrate concentration

d) Data insufficient
2) While carrying out a test in his lab, a scientist observed a following graph when he compared with his standard graphs. He could decipher that there is some kind of inhibition to its enzyme urease but could not determine the inhibition type. Please look at the graph and answer the questions given below to solve his mystery.

1. Inhibitor is causing which type of inhibition?
1. Uncompetitive
2. Competitive
3. Non- competitive
4. Mixed
2. What is the effect of inhibitor on Vmax of the reaction?
1. Same
2. Decrease
3. Increase
4. Data insufficient
3. What is the effect of inhibitor on the Km of the reaction?
1. Same
2. Decrease
3. Increase
4. Data insufficient

3)During the bioassay of enzyme urokinase test sample, some inhibition is observed. Please look at the graph and answer the questions given below to solve the mystery.

1. Inhibitor is of which type?
1. Uncompetitive
2. Competitive
3. Non- competitive
4. Mixed
2. What is the effect of inhibitor on Vmax of the reaction?
1. Same
2. Decrease
3. Increase
4. Data insufficient
3. What is the effect of inhibitor on the Km of the reaction?
1. Same
2. Decrease
3. Increase
4. Data insufficient

4)During the bioassay of enzyme oxidase test sample, some inhibition is observed. Please look at the graph and answer the questions given below to solve the mystery.

1. Inhibitor is of which type?
1. Uncompetitive
2. Competitive
3. Non- competitive
4. Mixed
2. What is the effect of inhibitor on Vmax of the reaction?
1. Same
2. Decrease
3. Increase
4. Data insufficient
3. What is the effect of inhibitor on the Km of the reaction?
1. Same
2. Decrease
3. Increase
4. Data insufficient